Rupali made her acting debut at the age of seven in the film Saaheb (1985) as a child actor.
Rupali appeared in the shows like Zara Nachke Dikha, Bigg Boss (2006), and Fear Factor – Khatron Ke Khiladi.
Rupali also played roles in the films ‘Mera Yaar Mera Dushman,’ ‘Angara,’ ‘Do Aankhen Barah Haath.’
In 2022, Rupali debut in a TV series ‘Sukanya’ and her role was appreciated.
Rupali acted in the series 'Dil Hai ki Manta Nahi,' wherein she played a double role. Her roles in the Star Plus series, 'Sanjivani' and 'Sarabhai versus Sarabhai' changed her career and life.
Rupali also worked in ‘Kkavyanjali’ and even in hit series such as ‘Baa Bahoo aur Baby,’ ‘Bidaai’ and ‘Kahaani Ghar Ghar ki,’ ‘Parvarish’ and ‘Adalat.’
In 2013, she take a break from TV series, and now she appeared in a famous TV serial "Anupama".
On 6 Feb 2013, Rupali Ganguly got married to her boyfriend, Ashwin K Verma and blessed with a baby boy in 2015.